Hyoscyamine 6β-hydroxylase, an enzyme involved in tropane alkaloid biosynthesis, is localized at the pericycle of the root
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منابع مشابه
Enhancing Tropane Alkaloid Production Based on the Functional Identification of Tropine-Forming Reductase in Scopolia lurida, a Tibetan Medicinal Plant
Scopolia lurida, a native herbal plant species in Tibet, is one of the most effective producers of tropane alkaloids. However, the tropane alkaloid biosynthesis in this plant species of interest has yet to be studied at the molecular, biochemical, and biotechnological level. Here, we report on the isolation and characterization of a putative short chain dehydrogenase (SDR) gene. Sequence analys...
متن کاملEnhanced secretion of tropane alkaloids in Nicotiana tabacum hairy roots expressing heterologous hyoscyamine-6beta-hydroxylase.
Hyoscyamine-6beta-hydroxylase (H6H; EC 1.14.11.11) catalyses oxidative reactions in the biosynthetic pathway leading from hyoscyamine to the more pharmaceutically valuable tropane alkaloid scopolamine. The h6h gene encoding H6H from Hyoscyamus niger was introduced, under the control of the CaMV 35S promoter, into non-hyoscyamine-producing Nicotiana tabacum and hyoscyamine-producing Hyoscyamus m...
متن کاملQuantitative and qualitative investigation of Duboisia myoporoides R.BR.
[1] Ziegler J, Facchini PJ (2008) Alkaloid Biosynthesis: Metabolism and Trafficking. Annu Rev Plant Biol 59:735–769. doi: 10.1146/annurev.arplant.59.032607.092730 [2] Averesch NJH, Kayser O (2014) Assessing Heterologous Expression of Hyoscyamine 6β-Hydroxylase – A Feasibility Study. Procedia Chem 13:69–78. doi: http://dx.doi.org/10.1016/j.proche.2014.12.008 [3] Kim, HK, Choi YH, Verpoorte R, (2...
متن کاملA small-scale proteomic approach reveals a survival strategy, including a reduction in alkaloid biosynthesis, in Hyoscyamus albus roots subjected to iron deficiency
Hyoscyamus albus is a well-known source of the tropane alkaloids, hyoscyamine and scopolamine, which are biosynthesized in the roots. To assess the major biochemical adaptations that occur in the roots of this plant in response to iron deficiency, we used a small-scale proteomic approach in which 100 mg of root tips were treated with and without Fe, respectively, for 5 days. Two-dimensional min...
متن کاملA Comprehensive Overview on Valuable Tropane Alkaloids: Scopolamine, Atropine, and Hyoscyamine
Tropane alkaloids such as scopolamine (C17H21NO4), atropine (C17H23NO3) and hyoscyamine (C17H23NO3) are the most important plant secondary metabolites in the pharmaceutical industry due to anticholinergic activity, competition with muscarinic receptors and also treating different human diseases. Scopolamine, hyoscyamine and atropine are the most important tropane alkaloids used as anticoagulant...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1991
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(20)64371-x